mass-spectrometry based sequenom massarray epityper platform  (Sequenom)

Journal: Environmental and molecular mutagenesis

Article Title: Bisphenol A-Associated Alterations in the Expression and Epigenetic Regulation of Genes Encoding Xenobiotic Metabolizing Enzymes in Human Fetal Liver

doi: 10.1002/em.21823

Figure Lengend Snippet: TABLE IV

Article Snippet: A standard HotStarTaq thermal cycling setting was used for the three assays with the following changes: CES2 required annealing at 56°C for 30 sec at 35 cycles, COMT required annealing at 60°C for 30 sec at 35 cycles, and SULT2A1 required annealing at 56°C for 30 sec at 40 cycles. table ft1 table-wrap mode=article t1 TABLE IV caption a4 Sequenom Epityper Primer Assays for DNA Methylation After in vitro transcription and uracil-cleavage of amplicons as previously described [ Coolen et al., 2007 ], promoter methylation was quantified using the mass-spectrometry based Sequenom MassArray EpiTYPER platform (Sequenom, San Diego, CA) in the University of Michigan DNA Sequencing Core.

Techniques: DNA Methylation Assay